Penicillinase Solution Composition and concentration The Penicillinase solution contains Storage and use by date The Penicillinase solution can be stored at 6 – 10 °C, until the mentioned date on the packing (for at least 6 weeks). In the case of contamination the solution may be rendered unusable or may lead to incorrect results. The solution must therefore always be kept clean and carried out with care. Instructions for use The Penicillinase solution can be used for the identification of penicillinase-unstable penicillins. The Penicillinase solution is delivered ready for use and can be used as follows:
1. Pipette 10 µl of the Penicillinase solution into one of the wells.
2. Add 0.1 ml of the positive test sample.
3. Incubate by the described manner. If the milk sample has been contaminated by
Penicillinase-unstable penicillins, the well will show a negative reaction after incubation. In the presence of other inhibitors, it will show a positive reaction.
Mechanism of action ß-lactam-antibiotics, like penicillins and cefalosporines can be inactivated by enzymes, the so-called ß-lactamases, to which also penicillinase belongs. ß-lactamases hydrolyse the lactam ring of the antibiotics, responsible for their activity. This ring can be protected by side chains against the ß-lactamases, as a result, the various ß-lactam-antibiotics have different stability. With the addition of penicillinase to a sample contaminated with penicillins the antibiotics are deactivated. So, during the test (BRT) the growth of the test germ Geobac. stearothermophilus var. calidolactis C953 (before B. stearothermophilus) is not inhibited any more and the test result is negative yellow cavity.
Penicillinase - Product Information, 04-2009.doc
Stability of selected beta-lactamase antibiotics against beta-lactamase
Substance Beta-lactamase Substance Beta-lactamase Stability Stability Penicillins Cefalosporines
Beta-lactamase does not inactivate other antiinfectives as e.g. tetracyclines, sulfonamides, makrolides or amino-glykosides
Further information BRT – Background and Basic Information BRT – Product Information BRT – Instructions for Use of Plates BRT – Instructions for Use of Tubes BRT – The Correct Incubation BRT – Use of Preserved Samples BRT – The Visual Evaluation BRT – The Photometric Evaluation BRT – Use of Milk Products Standards, lyophilisated – Product Information Penicillinase Product Information
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Penicillinase - Product Information, 04-2009.doc
Learning and Individual Differences 14 (2004) 137 – 151Working memory performance of Italian studentswith foreign language learning difficultiesa Department of Psychology, University of Pavia, Piazza Botta 6, 27100 Pavia, Italyb Department of Psychology, University of Padova, Padova, ItalyReceived 28 April 2003; received in revised form 10 November 2003; accepted 22 January 2004It has been
1 IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND OF THE COMPANY/UNDERTAKINGPowder component of a two component system. Mix with Liquid component before use. Versatile material for rebuilding, repairing and coating applications. Application by plastic applicator or spatula provided. Application by pouring. Please refer to the relevant Belzona® Instructions For Use for further information. For
Penicillinase Solution 
Stability of selected beta-lactamase antibiotics against beta-lactamase