Robert kevin blackburn

ROBERT KEVIN BLACKBURN


EXPERIENCE
Mass Spectrometry Laboratory Supervisor (Feb 2007 – Present)
Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC

Investigator (April 2001 – December 2006)
Disease and Biomarker Proteomics, GlaxoSmithKline, Inc., Research Triangle Park, NC

Research Scientist (August 1994-March 2001)
Department of Structural Chemistry, Glaxo Wellcome, Inc., Research Triangle Park, NC

Associate Scientist (April 1993-July 1994)
Department of Analytical Sciences, Glaxo, Inc., Research Triangle Park, NC

Graduate Research Assistant (January 1993-April 1993)
Department of Chemistry, North Carolina State University, Raleigh, NC

Teaching Assistant (January 1991-December 1992)
Department of Chemistry, North Carolina State University, Raleigh, NC

Science Teacher (August 1990-January 1991)
Clinton High School, Clinton, NC

AWARDS
Bronze Research Award, 2006 (Development of customized statistical tools for proteomics)
Exceptional Science Award, 2005 (Implementation of a label-free expression proteomics platform)
Bronze Research Award, 2004 (Development of a label-free expression proteomics platform)
Research Excellence Award, 1999 (Development of kinase substrate optimization technology)

EDUCATION
Master of Science, Analytical Chemistry (Minor in Biotechnology/Biochemistry), May 1993,
North Carolina State University, Raleigh, NC
Thesis: “Degradation of Cyclic Peptide Antibiotic Drugs by Immobilized Digestive Proteases” (Under the
direction of Professor Richard B. van Breemen)
Bachelor of Arts, Chemistry and Bachelor of Science, Science Education, Summa Cum Laude, May
1990, North Carolina State University, Raleigh, NC
PROFESSIONAL MEMBERSHIPS AND ACTIVITIES
American Society for Mass Spectrometry
ASMS Conference Program Review Committee, 2007 & 2008 ASMS Conference Short Course Instructor, 2001-present Triangle Area Mass Spectrometry Discussion Group
PUBLICATIONS
Kevin Blackburn, Fang-yi Cheng, John Williamson, and Michael B. Goshe. Data-Independent LC/MSE
Detection and Quantification of the Secreted Apium graveolens Pathogen Defense Protein Mannitol
Dehydrogenase. Rapid Commun. Mass Sp., 2010, 24 (7), 1009-1016.

Kevin Blackburn and Michael B. Goshe. Mass Spectrometry Bioinformatics: Tools for Navigating the
Proteomics Landscape. Curr. Anal. Chem., 2009, 5, 131-143.
Fang-yi Chien, Kevin Blackburn, Yu-Min Lin, Michael B. Goshe, and John Williamson. Absolute Protein
Quantification by LC/MSE for Global Analysis of Salicylic Acid-Induced Plant Protein Secretion
Responses. J. Prot. Res. 2009, 8, 82-93.
Kevin Blackburn. and Michael B. Goshe. Challenges and Strategies for Targeted Phosphorylation Site
Identification and Quantification using Mass Spectrometry Analysis. Briefings in Functional Genomics and
Proteomics, 2009, 8 (2), 90-103.
Jennifer Schaff, Flaubert Mbeunkui, Kevin Blackburn, David Mck. Bird, and Michael B. Goshe. SILIP:
A Novel Stable Isotope Labeling Method for In Plantae Quantitative Proteomic Analysis. The Plant
Journal, 2008, 56, 840-854.
Wang, X., Kota, U., He, K., Blackburn, K., Li, J., Goshe, M. B., Huber, S. C., and Clouse, S. D.
Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in
brassinosteroid signaling. Developmental. Cell, 2008, 15, 220-235.
M.H. Lambert, R.K. Blackburn, T.D.Seaton, D.B. Kassel, D.S. Kinder, M.A. Leesnitzer, D.M. Bickett,
J.R. Warner, M.W. Andersen, J.G.Badiang, D.J. Cowan, M.D. Gaul, K.G. Petrov, M.H. Rabinowitz, R.W.
Wiethe, J.D. Becherer, D.L. McDougald, D.L. Musso, R.C. Andrews, and M.L. Moss. Substrate
Specificity and Novel Selective Inhibitors of TNF-α Converting Enzyme (TACE) from Two-Dimensional
Substrate Mapping. Comb. Chem. High Throughput Screen. 2005, 8(4), 327-339.
Rina Plattner, Brenda J. Irvin, Shuling Guo, Kevin Blackburn, Andrius Kazlauskas, Robert T. Abraham,
John D. York, and Ann Marie Pendergast. A New Link Between The c-Abl Tyrosine Kinase and
Phosphoinositide Signaling Through PLC-γ1. Nature Cell Biol. 2003, 5, 309-319.
Wanda M. Bodnar, R. Kevin Blackburn, Jo M. Krise, and M. Arthur Moseley. Exploiting the
Complementary Nature of LC/MALDI/MS/MS and LC/ESI/MS/MS for Increased Proteome Coverage. J.
Am. Soc. Mass Spectrom. 2003, 14, 971-979.
Mohita J. Mohan, Theresa Seaton, Justin Mitchell, Anne Howe, Kevin Blackburn, William Burkhart,
Mary Moyer, Inder Patel, Gregory M. Waitt, J. David Becherer, Marcia L. Moss, and Marcos E. Milla.
The Tumor Necrosis Factor-α Converting Enzyme (TACE): A Unique Metalloproteinase with Highly
Defined Substrate Selectivity. Biochemistry. 2002, 41, 9462-9469.
Johannes P. C. Vissers, R. Kevin Blackburn, and M. Arthur Moseley. A Novel Interface for Variable
Flow Nanoscale LC/MS/MS for Improved Proteome Coverage. J. Am. Soc. Mass Spectrom. 2002, 13, 760-
771.
Lawrence E. Ostrowski, Kevin Blackburn, Kristin M. Radde, Mary B. Moyer, Daniela M. Schlatzer,
Arthur Moseley, and Richard C. Boucher. A Proteomic Analysis of Human Cilia: Identification of Novel
Components. Mol. and Cell. Proteomics, 2002, 1, 451-465.
Yan J. Zhang, Wanda K. O’Neal, Scott H. Randell, Kevin Blackburn, Mary B. Moyer, Richard C.
Boucher, and Lawrence E. Ostrowski. Identification of Dynein Heavy Chain 7 as an Inner Arm
Component of Human Cilia That is Synthesized but not Assembled in a Case of Primary Ciliary
Dyskinesia. J. Biol. Chem. 2002, 277, 17906-17915.
Gary K. Smith, David Barrett, Kevin Blackburn, Walter Dallas, Roderick Davis, Daniel Hassler, Randy
McConnell, Mary Moyer, and Kurt Weaver. Expression, Purification, High Throughput Screening and
Redox-Based Inhibition of Human Caspase-8. Archives Biochem. Biophysics. 2002, 399 (2), 195-205.
Perry S. Brignola, Karen Lackey, Sue Kadwell, Christine Hoffman, Earnest Horne, H. Luke Carter, J.
Darren Stuart, Kevin Blackburn, Mary B. Moyer, Krystal J. Alligood, Wilson B. Knight, and Edgar R.
Wood. Comparison of the Biochemical and Kinetic Properties of the Type-1 Receptor Tyrosine Kinase
Intracellular Domains: Demonstration of Differential Sensitivity to Kinase Inhibitors. J. Biol. Chem. 2002,
277 (2), 1576-1585.
Emine Cavdar Koc, William Burkhart, Kevin Blackburn, Mary B. Moyer, Daniela M. Schlatzer, Arthur
Moseley, and Linda L. Spremulli. The Large Subunit of the Mammalian Mitochondrial Ribosome:
Analysis of the Complement of Ribosomal Proteins Present. J. Biol. Chem. 2001, 276 (47), 43958-43969.
Emine Cavdar Koc, William Burkhart, Kevin Blackburn, Arthur Moseley, and Linda L. Spremulli. The
Small Subunit of the Mammalian Mitochondrial Ribosome: Identification of the Full Complement of
Ribosomal Proteins Present. J. Biol. Chem. 2001, 276(22), 19363-19374.
Emine Cavdar Koc, Asoka Ranasinghe, William Burkhart, Kevin Blackburn, Hasan Koc, Arthur Moseley,
and Linda L. Spremulli. A New Face on Apoptosis: Death-Associated Protein 3 (DAP3) and Programmed
Cell Death 9 Protein (PDC9 or p52) are Mitochondrial Ribosomal Proteins. FEBS Letters, 2001, 492, 166-
170.
Emine Cavdar Koc, William Burkhart, Kevin Blackburn, Hasan Koc, Arthur Moseley, and Linda L.
Spremulli. Identification of Four Proteins from the Small Subunit of the Mammalian Mitochondrial
Ribosome using a Proteomics Approach. Protein Science, 2001, 10, 471-481.
Derril Willard, Wen-Ji Chen, George Barrett, Kevin Blackburn, Jane Bynum, Thomas Consler, Christine
Hoffman, Earnest Horne, Marie A. Iannone, Sue Kadwell, Janet Parham, and Byron Ellis. Expression,
purification, and characterization of the human receptor activator of NF-κB ligand (RANKL) extracellular
domain. Protein Expression and Purification. 2000, 20(1), 48-57.
Ru Yang, Ram S. Puranam, Linda S. Butler, Wei-Hua Qian, Xiao-Ping He, Mary B. Moyer, Kevin
Blackburn, P. Ian Andrews, and James O. McNamara. Autoimmunity to Munc-18 in Rasmussen’s
Encephalitis. Neuron. 2000, 28, 375-383.
Su-Jun Deng, D. Mark Bickett, Justin L. Mitchell, Millard H. Lambert, R. Kevin Blackburn, H. Luke
Carter,III, Jennifer Neugebauer, Gregory Pahel, Michael P. Weiner, and Marcia L. Moss. Substrate
Specificity of Human Collagenase III Assessed Using a Phage Displayed Peptide Library. J. Biol. Chem.
2000, 275, 31422-31427.
Emine C. Koc, William Burkhart, Kevin Blackburn, Hasan Koc, and Linda L. Spremulli. A Proteomics
Approach to the Identification of Mammalian Mitochondrial Small Subunit Ribosomal Proteins. J. Biol.
Chem. 2000, 275(42), 32585-35291.
Brett S. Phinney, Kevin Blackburn, and Dennis T. Brown. The Surface Conformation of the Sindbis
Virus Glycoproteins E1 and E2 at Neutral and Low pH as Determined by Mass Spectrometry Based
Mapping. J. Virol., 2000, 74(12), 5667-5678.

Patricia A. Zipfel, Matthew Grove, Kevin Blackburn, Manabu Fujimoto, Thomas F. Tedder, and Ann
Marie Pendergast. The c-Abl Tyrosine Kinase is Regulated Downstream of the B Cell Antigen Receptor
and Interacts with CD19. J. Immunol. 2000 165(12), 6872-6879.
Emine C. Koc, Kevin Blackburn, William Burkhart, and Linda L. Spremulli. Identification of a
Mammalian Mitochondrial Homolog of Ribosomal Protein S7. Biochem. Biophys. Res. Commun. 1999,
266(1), 141-146.
Marcos E. Milla, M. Anthony Leesnitzer, Marcia L. Moss, William C. Clay, H. Luke Carter, Ann B. Miller,
Jui-Lan Su, Millard H. Lambert, Derril H. Willard, Douglas M. Sheeley, Thomas A. Kost, William
Burkhart, Mary Moyer, R. Kevin Blackburn, Gregory L. Pahel, Justin L. Mitchell, Christine R. Hoffman,
and J. David Becherer. Specific sequence elements are required for the expression of functional tumor
necrosis factor-{alpha}-converting enzyme (TACE). J. Biol. Chem. 1999, 274, 30563-30570.
R. Kevin Blackburn and M. Arthur Moseley, III. Quadrupole Time-of-Flight Mass Spectrometry: A
Powerful New Tool for Protein Identification and Characterization. Am. Pharm. Rev. 1999, 2, 49-59.
Monireh Roghani, J. David Becherer, Marcia L. Moss, Ruth E. Atherton, Hediye Erdjument-Bromage,
Joaquin Arribas, R. Kevin Blackburn, Gisela Weskamp, Paul Tempst, and Carl P. Blobel.
Metalloprotease-Disintegrin MDC9: Intracellular Maturation and Catalytic Activity. J. Biol. Chem. 1999,
274, 3531-3540.
R. Kevin Blackburn and Robert J. Anderegg. Characterization of Femtomole Levels of Proteins in
Solution Using Rapid Proteolysis and Nanoelectrospray Ionization Mass Spectrometry. J. Am. Soc. Mass
Spectrom. 1997, 8, 483-494.
Robert J. Anderegg, David S. Wagner, R. Kevin Blackburn, Gregory J. Opiteck, and James W. Jorgenson.
A Multidimensional Approach to Protein Characterization. J. Protein Chem. 1997, 16(5), 523-526.
R. Kevin Blackburn and Richard B. van Breemen. Application of an Immobilized Digestive Enzyme
Assay to Measure Chemical and Enzymatic Hydrolysis of the Cyclic Peptide Antibiotic Lysobactin. Drug
Metab. Disp. 1993, 21, 573-579.
BOOK CHAPTERS
Daniel B. Kassel, R. Kevin Blackburn, and Bruno Antonsson. “Strategies for the Rapid Characterization
of Recombinant and Native Proteins by Mass Spectrometry” in Mass Spectrometry of Biological Materials,
2nd Edition; Larsen, B. S. and McEwen, C. N., Eds.; Marcel Dekker: New York, 1998.
M. Arthur Moseley, Douglas M. Sheeley, R. Kevin Blackburn, Robert L. Johnson, and Barbara M.
Merrill. “Mass Spectrometry in Drug Discovery” in Mass Spectrometry of Biological Materials, 2nd
Edition; Larsen, B. S. and McEwen, C. N., Eds.; Marcel Dekker: New York, 1998.
PRESENTATIONS
R. Kevin Blackburn, M. Arthur Moseley, and Douglas M. Sheeley. Co-instructor of the “Mass
Spectrometry of Peptides and Proteins” 2-day short course taught annually at the American Society for
Mass Spectrometry Conference; served as co-instructor of this course since its inception in 2001.
John D. Williamson, Fang-yi Cheng, Eli Zamski, Kevin Blackburn, Wei-wen Guo, Mason Pharr, and
Michael B. Goshe. “Salicylic Acid Stimulates Secretion of the Typically Cytosolic Metabolic Enzyme
Mannitol Dehydrogenase”, presented at the Plant Biology 2009 meeting, Honolulu, Hawaii, 2009.
Xiaofeng Wang, Uma Kota, Kevin Blackburn, Karen Browning, Michael B. Goshe, and Steven D.
Clouse. “Receptor Kinases Involved in Brassinosteroid Signal Transduction Phosphorylate Protein
Translation Initiation Factors”, presented at the Plant Biology 2009 meeting, Honolulu, Hawaii, 2009.
Kevin Blackburn, Fang-yi Cheng, John Williamson, and Michael B. Goshe. “Detection and
Quantification of a Novel Plant Pathogen Defense Protein Mannitol Dehydrogrenase”, presented at The
57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA, 2009.
Uma Kota, Kevin Blackburn, Steven D. Clouse, and Michael B. Goshe. “Data-Independent Relative
Quantification of Salt Stress-Induced Membrane Protein Abundance Changes in Arabidopsis thaliana
Using Label-Free GeLC/MSE”, presented at The 57th ASMS Conference on Mass Spectrometry and Allied
Topics, Philadelphia, PA, 2009.
Ko-yi Chien, Kevin Blackburn, Hsiao-Ching S. Liu, and Michael B. Goshe. “Phosphopeptide Analysis
of Marek’s Disease Virus (MDV)-Infected Cells Using Electrostatic Repulsion Hydrophilic Interaction
Chromatography (ERLIC), IMAC, and LC/MS/MS”, presented at The 57th ASMS Conference on Mass
Spectrometry and Allied Topics, Philadelphia, PA, 2009.
Kevin Blackburn. “Applications of Data-Independent LC/MSE for the Characterization of Plant Proteins
and Proteomes”, presented at a Waters Corporation sponsored symposium held at the University of
Pennsylvania School of Medicine, Philadelphia, PA, 2008.
Mitra, S. K., Walters, B. T., Blackburn, K., Goshe, M. B., and Clouse, S. D. “An Organic Solvent-Based
Extraction Method for the Arabidopsis Membrane Subproteome and its Application in Quantitative
Proteomics”, presented at The Meeting of the American Society of Plant Biologists, Mérida, Mexico, 2008.
Blackburn, K. and Goshe, M. B. “Application of Data-Independent Parallel Fragmentation for Label-Free
Proteomic Analysis and Protein Characterization”, presented at The 56th ASMS Conference on Mass
Spectrometry and Allied Topics, Denver, CO, 2008.
Cheng, F-Y., Blackburn, K., Williamson, J., and Goshe, M. B. “Global Analysis of Pathogen-Induced
Plant Protein Secretion Responses Using Label-Free Quantification”, presented at The 56th ASMS
Conference on Mass Spectrometry and Allied Topics, Denver, CO, 2008.
Mbeunkui, F., Schaff, J. E., Blackburn, K., Bird, D. McK., and Goshe, M. B. “SILIP: A Novel Method for
Stable Isotope Labeling of Proteins In Planta for Quantitative Proteomic Analysis”, presented at The 56th
ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, 2008.
Kota, U., Blackburn, K., Mitra, S. K., Walters, B. T., Clouse, S. D., and Goshe, M. B “Label-Free
Quantification of Hormone-Induced Membrane Protein Abundance Changes in Arabidopsis thaliana”,
presented at The 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, 2008.
Ramaroson, M. F., Blackburn, K., Liu, H-C. S., and Goshe, M. B. “Using Label-Free Quantification to
Assess Sample Preparation: Application to Protein Expression Dynamics of Marek’s Disease Viral
Infection”, presented at The 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver,
CO, 2008.
Kevin Blackburn. “Applications of MSE in Plant Proteomics and Protein Characterization”, presented at a
symposium entitled “Label-Free Quantification and Identification Proteomics”, sponsored by the
Massachusetts Institute of Technology Department of Chemical Engineering, Cambridge, MA, 2008.
Rohina Amiri, John Gantt, Kevin Blackburn, and Michael B. Goshe. “Developing Effective Mass
Spectrometry Sample Preparation Methods for Enhanced Sequence Coverage of Proteins: Evaluating the
Use of Alternative Chemical Denaturants”, presented at The Annual Biomedical Research Conference for
Minority Students (ABRCMS), Austin, TX, 2007.
Laura Dubois, Deidre Dalmas, Marshall Scicchitano, Daniela Schlatzer, Mary Moyer, Jack Liu, Arthur
Moseley, Neal Cariello, Marlene Darfler, and Kevin Blackburn. “Biomarker Proteomics from Formalin-
Fixed Paraffin-Embedded Liver and Breast Tissue Sections”, presented at the 55th ASMS Conference on
Mass Spectrometry and Allied Topics, Indianapolis, IN, 2007.

Arthur Moseley, Kevin Blackburn, Dan Crowther, Scott Geramonos, Keith Richardson, Daniela Schlatzer,
Jeff Silva, and Dan Wall. "Peptide Mass Mapping – Qualitative and Quantitative Global Proteomic
Studies", presented at the Sixth Siena Meeting, From Genome to Proteome: Biomarker Discovery &
Imaging Proteomes, Siena, Italy, 2004.
Mary Moyer, Kevin Blackburn, Wanda Bodnar, Roderick Davis, Jo Krise, Daniela Schlatzer, and Arthur
Moseley. "Targeted Quantitative Protein Analyses Using Multiple Reaction Monitoring”, presented at the
52nd ASMS Conference on Mass Spectrometry and Allied Topics, Nashville, TN, 2004.
John Walsh, J., Reese, M., Burke, T.W., Moyer, M., Burkhart, W., Davis, R., Blackburn, R.K., and D.
Schlatzer. “Protein Targets of reactive metabolites of Abacavir (Ziagen) in the Rat”, presented at the
International Society for the Study of Xenobiotics (ISSX), Vancouver, Canada, 2004.
Daniela Schlatzer, Kevin Blackburn, Jo Krise, and Arthur Moseley. "Strategies for MS Westerns Using a
Quadrupole Time-of-Flight Mass Spectrometer", presented at the Sixth International Symposium on Mass
Spectrometry in the Health and Life Sciences: Molecular and Cellular Proteomics, San Francisco, CA,
2003.
Daniela Schlatzer, John Armstrong, Kevin Blackburn, William Burkhart, Roderick Davis, Alex Iving,
Jungping Jing, Sue Kadwell, Joanna Krise, Mary Moyer, Arthur Moseley, Kieran Todd, James Ward, and
Patrick Warren. “LC/LC/MS/MS Analysis of a Complex Proteome and Protein Classification by Gene
Ontology”, presented at The 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando,
FL, 2002.
Mark McDowall, Jeff Brown, Kevin Blackburn, Dominic Gostick, Arthur Moseley, Richard Tyldesley,
and Jim Langridge. “The Separation of Complex Tryptic Peptide Mixtures by Nanobore HPLC Combined
with Automated Eluent Collection onto High-Density Arrays for MALDI MS and MS/MS Analyses”,
presented at The Fifth International Symposium on Mass Spectrometry in the Health and Life Sciences:
Molecular and Cellular Proteomics, San Francisco, CA, 2001.
R. Kevin Blackburn, Lawrence E. Ostrowski, William A. Burkhart, Mary B. Moyer, Daniela M.
Schlatzer, Kristin M. Radde, Richard C. Boucher, and M. Arthur Moseley. “A Comprehensive Proteomic
Analysis of Human Cilia: Identification of Novel Ciliary Components”, presented at The 49th ASMS
Conference on Mass Spectrometry and Allied Topics, Chicago, IL, 2001.
William Burkhart, Emine Koc, Kevin Blackburn, Mary Moyer, Daniela Schlatzer, Arthur Moseley, and
Linda Spremulli. “The Mammalian Mitochondrial Ribosome: Towards Identification of the Full
Complement of Ribosomal Proteins Present”, presented at The 49th ASMS Conference on Mass
Spectrometry and Allied Topics, Chicago, IL, 2001.
Arthur Moseley, Kevin Blackburn, William Burkhart, Roderick Davis, Mary Moyer, Daniela Schlatzer,
Jim Langridge, Richard Tyldesley, Gary Schultz, Thomas Corso, Simon Prosser, and Jack Henion.
“Contrapuntal Methods for Improving Proteome Coverage with Nanoscale Capillary LC/MS/MS:
Variations on a Theme”, presented at The 49th ASMS Conference on Mass Spectrometry and Allied Topics,
Chicago, IL, 2001.
Mary Moyer, Kevin Blackburn, William Burkhart, Arthur Moseley, Lawrence Ostrowski, and Richard
Boucher. “A Comprehensive Proteomic Analysis of Human Cilia Using Nanoscale Capillary LC/MS/MS”,
presented at The Association of Biomolecular Resource Facilities Meeting, San Diego, CA, 2001.
Kevin Blackburn, Emine Koc, William Burkhart, Mary Moyer, Arthur Moseley, and Linda Spremulli.
“Identification of Mammalian Mitochondrial Ribosomal Proteins by Nanoscale Capillary LC/MS/MS”,
presented in the New Objective Users Meeting at The Association of Biomolecular Resource Facilities
Meeting, San Diego, CA, 2001.
Kevin Blackburn, William Burkhart, Roderick Davis, Mary Moyer, and Arthur Moseley. “Improved
Proteome Coverage Through the Use of Affinity Tags with Nanoscale Capillary LC/MS/MS”, presented at
The Association of Biomolecular Resource Facilities Meeting, San Diego, CA, 2001.
M. Arthur Moseley, Kevin Blackburn, and J. P. C. Vissers. “Automated Protein Identification using
Variable Flow Nanoscale Capillary Chromatography Coupled to a Q-TOF Mass Spectrometer”, presented
at The British Mass Spectrometry Miniaturization Meeting, Harlow, UK, 2000.
William Holmes, Kevin Blackburn, William Burkhart, Thomas Consler, Eric Dixon, Christine Hoffman,
Bradley McDonald, Mary Moyer, and Jeff Hutchins. “Purification and Characterization of a c-fms
Protease-Defined Kinase Domain”, presented at the Fourteenth Symposium of the Protein Society, San
Diego, CA, 2000.
William Burkhart, Kevin Blackburn, Arthur Moseley, Emine Koc, Hasan Koc, and Linda Spremulli.
“Proteomic Approaches for Identifying Components of the Bovine Mitochondrial Small Subunit (28S)
Complex”, presented at the Fourteenth Symposium of the Protein Society, San Diego, CA, 2000.
Derril Willard, Wen-Ji Chen, Kevin Blackburn, Jane Bynum, Thomas Consler, Earnest Horne, Sue
Kadwell, and Byron Ellis. “Biochemical characterization and determination of the quaternary structure of
the human receptor activator of NF-κB ligand (RANKL) extracellular domain”, presented at the Fourteenth
Symposium of the Protein Society, San Diego, CA, 2000.
Patricia A. Zipfel, Matthew P. C. Grove, R. Kevin Blackburn, Manabu Fujimoto, Thomas F. Tedder, and
Ann Marie Pendergast. “Role of c-Abl in B Cell Receptor Signaling”, presented at a symposium entitled
Tyrosine Phosphorylation and Cell Signaling: The Third Decade, La Jolla, CA, 2000.
Kevin Blackburn, William Burkhart, Arthur Moseley, Emine Koc, Hasan Koc, and Linda Spremulli.
“Proteomic Approaches for Identifying Components of the Mammalian Mitochondrial Small Subunit (28S)
Complex”, presented at the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach,
CA, 2000.
Arthur Moseley, Kevin Blackburn, William Burkhart, Hans Vissers, and Robert Bordoli. “Increased
Proteome Coverage Using a Novel Peak Trapping Interface for Nanoscale Capillary LC/MS/MS Analysis
of Complex Peptide Mixtures”, presented at the 48th ASMS Conference on Mass Spectrometry and Allied
Topics, Long Beach, CA, 2000.
Douglas M. Sheeley, Kevin Blackburn, and Arthur Moseley. “Detailed Characterization of Protein
Glycosylation Using a Combined Approach of Nanoscale Data-Dependent LC/MS/MS and MS/MS of
Methylated Oligosaccharides”, presented at the 48th ASMS Conference on Mass Spectrometry and Allied
Topics, Long Beach, CA, 2000.
Brett S. Phinney, Kevin Blackburn, and Dennis T. Brown. “The Solution Structure of the Sindbis Virus at
Neutral and Low pH as Assayed by Mass Spectrometry Mass Mapping and Selective Chemical
Modification”, presented at the Association of Biomolecular Resource Facilities Meeting, Bellevue, WA,
2000.
M.A. Moseley, J. M. Neugebauer, and R.K. Blackburn. “Fully Automated Nanoscale Capillary
LC/MS/MS using a Hybrid Quadrupole/Time-of-Flight Mass Spectrometer”, presented at The Sixteenth
Montreux Symposium on LC/MS, Hilton Head, SC, 1999.
M.A. Moseley, J.N. Neugebauer, and R.K. Blackburn. "The Role of Nanoscale Capillary LC/MS/MS in
Proteomics”, presented at The Fifteenth Asilomar Conference on Mass Spectrometry, “Mass Spectrometry
and Proteomics”, Pacific Grove, CA, 1999.
Patricia A. Zipfel, Matthew P. C. Grove, Kevin Blackburn, Manabu Fujimoto, Thomas F. Tedder, and
Ann Marie Pendergast. “Role of c-Abl Downstream of the B-cell Receptor”, presented at the FASEB
Summer Conference: Protein Kinases and Phosphorylation, Snowmass, CO, 1999.
R.K. Blackburn. “Routine Microscale Sample Preparation Techniques for Protein Identification and
Characterization”, American Society for Mass Spectrometry Fall Workshop entitled “Microscale Sample
Preparation for Peptide and Protein Analysis”, Philadelphia, PA, November, 1999.

Kevin Blackburn and M. Arthur Moseley. “Protein Identification and Characterization Using a
Quadrupole Time-of-Flight Mass Spectrometer”, presented at the 37th Eastern Analytical Symposium and
Exposition, Somerset, NJ, 1998.
Kevin Blackburn. “Protein Identification and Characterization Using Nanoelectrospray Ionization Mass
Spectrometry”, presented at The 50th Southeastern Regional Meeting of the American Chemical Society,
Research Triangle Park, NC, 1998.
Kevin Blackburn and Robert J. Anderegg. “Protein Micro-Sample Preparation for MALDI and
Nanoelectrospray Ionization MS”, presented at the 46th ASMS Conference on Mass Spectrometry and
Allied Topics, Orlando, FL, 1998.
Robert J. Anderegg, Kevin Blackburn, Roderick Davis, Arthur Moseley, Mary Moyer, and Douglas
Sheeley. “Protein Identification by In-Gel Digestion and Mass Spectrometry: Synergy Between MALDI-
TOFMS and Nanoelectrospray”, presented at The 46th ASMS Conference on Mass Spectrometry and Allied
Topics, Orlando, FL, 1998.
Robert J. Anderegg and R. Kevin Blackburn. “Large and Small Molecule Applications of
Nanoelectrospray Ionization Mass Spectrometry”, presented at The Triangle Area Mass Spectrometry
Discussion Group Meeting, Research Triangle Park, NC , 1997.
R. Kevin Blackburn and Robert J. Anderegg. “Practical Considerations of Protein Identification Through
the Use of Peptide Sequence Tags”, presented at The 45th ASMS Conference on Mass Spectrometry and
Allied Topics, Palm Springs, CA, 1997.
R. Kevin Blackburn, Marcia Moss, Daniel Kassel, Millard Lambert, and Dan Kinder. “Mapping of TNFα
Converting Enzyme Substrates by Decoding Peptide Libraries Using Two-Dimensional HPLC/MS”,
presented at the 45th ASMS Conference on Mass Spectrometry and Allied Topics, Palm Springs, CA, 1997.
Philippe A. Guy, Neal Gordon, R. Kevin Blackburn, and Robert J. Anderegg. “Development of a
Coupling Method Between the Integral Workstation and ESIMS to Study Protein Conformation”,
presented at The 45th ASMS Conference on Mass Spectrometry and Allied Topics, Palm Springs, CA,
1997.
D. Willard, A. Payne, K. Blackburn, W. Burkhart, W. Clay, E. Furfine, A. Hassell, C. Hoffman, L. Miller,
M. Moyer, L. Shewchuk, and T. Consler. “A Comparison of the Kinetic and Biophysical Characteristics of
the Human and Rat Homologs of Cathepsin K”, presented at The Protein Society Tenth Symposium,
Boston, MA, 1997.
Su-Jun Deng, R. Kevin Blackburn, D. Mark Bickett, H. Luke Carter, Justin L. Mitchell, Gregory Pahel,
Michael P. Weiner, and Marcia L. Moss. “Probing Substrate Specificity of Human Collagenase-3 (MMP-
13) by Phage Display”, presented at the Display Technologies in Protein Engineering, Drug Discovery,
Molecular Evolution, and Vaccine Development Meeting, Lake Tahoe, NV, 1997.
Kevin Blackburn and Rob Anderegg. “Combining Rapid Proteolysis with Nanoelectropsray Ionization
for Protein Characterization”, presented at The 44th ASMS Conference on Mass Spectrometry and Allied
Topics, Portland, OR, 1996.

Kevin Blackburn, Daniel Kassel, Gaochao Tian, and Blaine Knight. “Optimization of pp60c-src Substrates
by Decoding Peptide Libraries Using LC/MS”, presented at The 44th ASMS Conference on Mass
Spectrometry and Allied Topics, Portland, OR, 1996.
R. K. Blackburn, J. Mark, and D. B. Kassel. “In-situ Chemical Modification, Digestion, and
Characterization of Proteins Utilizing a 2-D HPLC/MS Approach”, presented at the 43rd ASMS Conference
on Mass Spectrometry and Allied Topics, Atlanta, GA, 1995.
Richard B. van Breemen and R. Kevin Blackburn. “Hydrolysis of Peptide and Protein Drugs by
Immobilized Hepatic Proteases Measured Using Continuous-Flow FAB LC/MS and MS/MS”, presented at
The 41st ASMS Conference on Mass Spectrometry and Allied Topics, San Francisco, CA, 1993.
R. Kevin Blackburn and Richard B. van Breemen. “Continuous-Flow Fast Atom Bombardment Mass
Spectrometry of Enzymatic Hydrolysis Products of Cyclic Peptide Drugs”, presented at The 40th ASMS
Conference on Mass Spectrometry and Allied Topics, Washington, DC, 1992.
PATENT APPLICATIONS
Robert Kevin Blackburn, William Alfred Burkhart, Steve A Haneline, Wayne Howard Miller, Mary
Benbow Moyer, David John Porter, Steven A Short. “A novel deaminase enzyme capable of catalyzing
conversion of the monophosphorylated form of a purine nucleoside analogue, and methods of using such
enzyme”, filed May, 2002.
Robert Kevin Blackburn, Harold Neal Bramson, Mary Benbow Moyer, James Darren Stuart. “Protein
Kinase Peptide Substrate Optimization Using Peptide Libraries”, filed May, 2002.

Source: http://biochem.ncsu.edu/MassSpec/docs/KevinBlackburn-CV.pdf