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Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight The Effect of Chlorogenic Acid Enriched
Coffee (Green Coffee for Slimmers) on
Weight When Used in Overweight People
An independent market research, comparative, randomized, double-blind, 4 week study on 62 healthy volunteers using instant coffee enriched with chlorogenic acid – Green Coffee for Slimmers – compare with normal instant coffee show that the average losses in weight in the chlorogenic acid enriched coffee was 1.35kg and 0.12kg in the normal instant coffee. These results confirmed prior medical research on the effect of Chlorogenic Acid Enriched Coffee published in the Journal of International Medical Research, 2007; 35:900 -908, which concluded that “we conclude that chlorogenic acid enriched instant coffee appears to have a significant effect absorption and utilization of glucose from the diet. This effect, if the coffee is used for an extended time, may result in reduced body mass and body fat when compared with the use of normal instant coffee.”. This study reveals that 75% of the volunteers drinking Green Coffee for Slimmers lost on average 1.60 kg over 2 weeks and 80% of the volunteers lost on average 2.02 kg over 4 weeks. KEY WORDS: Chlorogenic Acid; Coffee; Weight Loss
Introduction
Coffee is an extremely rich source of chlorogenic acids. The amounts of chlorogenic acids and caffeine in coffee are comparable.² Chlorogenic acids are an important group of biologically active dietary phenols; the best known being 5-caffeoylquinic acid. The daily intake of chlorogenic acids by coffee drinkers is considered to be in the range 0.5 – 1.0 g ² ³ and chlorogenic acids have been found to exhibit antioxidant activity in vitro³. A previous study has shown that chlorogenic acid supplemented coffee may reduce body mass and body fat when compared with the use of instant coffee. The importer of Green Coffee for Slimmers in the EU - commissioned an
independent market research study carried out by Ayton Global Research, UK.
The purpose of the study was to assess the weight loss results capability of Green
Coffee for Slimmers in a comparative, double-blind study, 4-week study on 62
volunteers.
1 Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight The overall aim of the study was to provide quantifiable and unbiased factual evidence that Green Coffee for Slimmers is an effective „weight loss support tool‟ even when an
individual does not change their diet
.
What is Green Coffee for Slimmers?
Green Coffee for Slimmers is a food supplement containing green coffee extract. Each sachet of Green Coffee for Slimmers contains 6500mg of Coffea Arabica. Green Coffee for Slimmers is a chlorogenic acid enriched coffee (5.85%), equivalent to 175.5mg of chlorogenic acid. The recommended daily dosage is one cup of Green Coffee for Slimmers only, before breakfast. Materials and methods
Products Used
The test coffee product used in this study was Green Coffee for Slimmers, packed in sachets each containing 10000 mg of instant coffee. A total of 6500 mg per 10000 mg of the Green Coffee for Slimmers product comprises an extract of green coffee, obtained by a traditional process, from the beans of Coffea Arabica. This is particularly rich in chlorogenic acids (45-50%). The extraction process is performed with alcohol as the solvent and the extract contains <2% of caffeine. Nescafé® Expresso Pur Arabica Original was used as the comparative product. The literature indicates that instant coffees contain 30 -40 mg/g of chlorogenic acid. The study was carried out to evaluate, over a period of 4 weeks, the effect of drinking Green Coffee for Slimmers compared with the effect of drinking normal instant coffee on weight when taken as part of a regular diet in slight to moderately overweight volunteers. Volunteer recruitment, study design and treatment
The volunteers recruited through Ayton Global Research were slightly to moderately overweight (BMI 25 – 30) non-smokers not taking any drugs on a regular basis for the treatment of chronic disease. All the volunteers were asked to follow the same lifestyle that they had prior to entering the study and not to commence other weight loss programmes whilst participating in the study. They did not receive any information on diet and were also told to maintain their current physical exercise. A total of 62 volunteers were selected as part of this study. 2 Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight The study was designed as a randomised, placebo-controlled study. Half of the volunteers drank Green Coffee for Slimmers [one cup(sachet)/ day (10g/day of coffee) while the other half drank normal Nescafé® Expresso Pur Arabica Original blend instant coffee. The duration of the study was 4 weeks. The weight, waist, bust and hips were measured initially and once a week. Which global research firm conducted the research?
Ayton Global Research, based in the UK managed the independent study, using appropriate protocols. Ayton Global Research (AGR) (www.aytonresearch.co.uk) was originally established in 1995 by Sue Ayton to provide consumer trials for the cosmetics and toiletries industry. It is now proud to offer comprehensive consumer research in many business areas, but predominantly Cosmetics and Food and Drink. Statistical analysis
Data were presented as means ± SD. Student‟s t-test (parametric test) was used to compare differences between values. Differences with a P-value < 0.05 was considered to be statistically significant. Data was verified to validate it was following a Gaussian curve using Skewness and Kurtosis parameters. 62 volunteers (BMI 25-30) were enrolled in this study; 30 received Green Coffee for Slimmers™ and 32 received the normal instant coffee (Nescafé® Expresso Pur Arabica Original). Table 1 presents the measurements for the two groups of participants at the start of study with respect to the parameters of interest. Results from the weight study (table 2) show that the mean ±SD weight reductions for Green Coffee for Slimmers and normal instant coffee drinkers were 1.35 ± 0.81 and 0.12 ± 0.27 kg, respectively, over a 4 week period. Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight TABLE 1:
Mean ± SD anthropometric parameters for overweight volunteers taking Green
Coffee for Slimmers (total 10g/day) or Nescafé® Expresso Pur Arabica Original
instant coffee (total 3g/day) for 4 weeks.
No. of volunteers
Start weight (Kg)
TABLE 2:
Mean ± SD weight for overweight volunteers taking Green Coffee for Slimmers™
(total 10g/day) or Nescafé® Expresso Pur Arabica Original instant coffee (total
3g/day) for 4 weeks.
Weight (Kg)
Difference
Results from the waist study (table 3 and 4) show that the mean ±SD waist reductions for Green Coffee for Slimmers and normal instant coffee drinkers were 2.77 ± 1.65 and 0.02 ± 0.27 cm, respectively, over a 4 week period. TABLE 3:
Mean ± SD anthropometric parameters for overweight volunteers taking Green
Coffee for Slimmers (total 10g/day) or Nescafé® Expresso Pur Arabica Original
instant coffee (total 3g/day) for 4 weeks.
No. of volunteers
Start waist (cm)
Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight TABLE 4:
Mean ± SD waist measurement for overweight volunteers taking Green Coffee for
Slimmers™ (total 10g/day) or Nescafé® Expresso Pur Arabica Original instant coffee
(total 3g/day) for 4 weeks.
Waist (cm)
Difference
Results from the hips study (table 5 and 6) show that the mean ±SD hips reductions for Green Coffee for Slimmers and normal instant coffee drinkers were 2.05 ± 1.65 and 0.02 ± 0.41 cm, respectively, over a 4 week period. TABLE 5:
Mean ± SD anthropometric parameters for overweight volunteers taking Green
Coffee for Slimmers (total 10g/day) or Nescafé® Expresso Pur Arabica Original
instant coffee (total 3g/day) for 4 weeks.
No. of volunteers
Start hips (cm)
TABLE 6:
Mean ± SD hips measurement for overweight volunteers taking Green Coffee for
Slimmers™ (total 10g/day) or Nescafé® Expresso Pur Arabica Original instant coffee
(total 3g/day) for 4 weeks.
Hips (cm)
Difference
Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight Results from the bust study (table 7 and 8) show that the mean ±SD bust reductions for Green Coffee for Slimmers and normal instant coffee drinkers were 1.42 ± 0.72 and 0.37 ± 0.46 cm, respectively, over a 4 week period. TABLE 7:
Mean ± SD anthropometric parameters for overweight volunteers taking Green
Coffee for Slimmers (total 10g/day) or Nescafé® Expresso Pur Arabica Original
instant coffee (total 3g/day) for 4 weeks.
No. of volunteers
Start bust (cm)
TABLE 8:
Mean ± SD bust measurement for overweight volunteers taking Green Coffee for
Slimmers™ (total 10g/day) or Nescafé® Expresso Pur Arabica Original instant coffee
(total 3g/day) for 4 weeks.
Hips (cm)
Difference
Conclusion:
For the Green Coffee for Slimmers drinkers, over a 4 weeks period the weight loss was on average 2.02 kg (range 0.12 to 5.53 kg); 4.87 cm around the waist (range 1.27 to 15.24 cm); 3.27 cm around the hips (range 1 – 7.62 cm) and 3.16 cm around the bust ( range 0.5 – 10.16 cm). Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight TABLE 9:
Green Coffee for Slimmers™ average, min and max weight measurements for 4
weeks for the Green Coffee for Slimmers drinker who lost weight.
Green Coffee for Slimmers Weight Loss (Kg)
TABLE 10:
Nescafé® Expresso average, min and max weight measurements for 4 weeks for the
Nescafé® drinker who lost weight.
Nescafé Weight Loss (Kg)
TABLE 11:
Green Coffee for Slimmers™ average, min and max waist measurements for 4 weeks
for the Green Coffee for Slimmers drinker who lost weight.
Green Coffee for Slimmers Waist Loss (cm)
TABLE 12:
Nescafé® Expresso average, min and max waist measurements for 4 weeks for the
Nescafé® drinker who lost weight.
Nescafé Waist Loss (cm)
Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight TABLE 13:
Green Coffee for Slimmers™ average, min and max hips measurements for 4 weeks
for the Green Coffee for Slimmers drinker who lost weight.
Green Coffee for Slimmers Hips Loss (Kg)
TABLE 14:
Nescafé® Expresso average, min and max hips measurements for 4 weeks for the
Nescafé® drinker who lost weight.
Nescafé Hips Loss (Kg)
TABLE 15:
Green Coffee for Slimmers™ average, min and max bust measurements for 4 weeks
for the Green Coffee for Slimmers drinker who lost weight.
Green Coffee for Slimmers Bust Loss (cm)
TABLE 16:
Nescafé® Expresso average, min and max bust measurements for 4 weeks for the
Nescafé® drinker who lost weight.
Nescafé Bust Loss (cm)
A number in () indicates a weight gain, a waist gain, a hips gain or bust gain. Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010 Ayton Global Research – Independent Market Study on the effect of Green Coffee for Slimmers on weight A statistical analysis confirmed that 95% of the values follow a Gaussian curve (skewness and kurtosis values are between -2 and +2). The difference in weight loss between the groups was statistically significant (P < 0.05) and the weight loss in the Green Coffee for Slimmers group by the end of the study was also statistically significant. Finally, 75% of the volunteers (table 16) on Green Coffee for Slimmers lost an
average of 1.60 kg over a 2 week period and 80% of the volunteers lost on
average 2.02 kg over a 4 week period.

TABLE 17:
Percentage of volunteers who have lost weight using Green Coffee for Slimmers™ for
4 weeks for the Green Coffee for Slimmers drinker who lost weight.
Green Coffee for Slimmers Percentage Volunteers with weight Loss (%)
References:
1 Greenberg JA, Axen KV, Schnoll R, et al: Coffee, tea and diabetes: the role of weight loss and caffeine. Int J Obesity 2005, 29: 1121-1129 2 Clifford MN: Chlorogenic acids and other cinnamates – nature, occurrence, dietary burden, absorption and metabolism. J Sci Food Agric 2000; 80:1033-1043 3 Olthof MR, Hollman PCH, Katan MB: Chlorogenic acid and caffeic acid are absorbed in humans. J Nutr 2000; 131:66-71 4 E. Thom: The effect of Chlorogenic Acid Enriched Coffee on Glucose Absorption in Healthy volunteers and its effect on Body Mass When Used Long-Term in Overweight and Obese People. J Int Medical Research 2007:35: 900-908 5 Johnston KL, Clifford MN, Morgan LM: Coffee acutely modifies gastrointestinal hormone secretion and glucose tolerance in humans: glycemic effexts of chlorogenic acid and caffeine. Am J Clin Nutr 2003; 78:728-733. Results of Independent Market Research Study on Green Coffee for Slimmers – June 2010

Source: http://www.crudocaffe.it/studi/chlorogenic-effect.pdf

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3rd International and 18th National Congress of Iranian Society for Reproductive Medicine 18-20 April 2012 Tabriz-Iran Poster Approved 1 A comparative Study of the quality of life between man and woman in infertile couples A comparing between differentiation process and Reproductive cloning A Comparison between Semen Parameters Before and After Swim Up in Intrauterine Insemination me

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