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Progesterone ELISA Kit
Enzyme immunoassay kit for the quantitative determination of Human Progesterone concentration in serum. For in vitro diagnostic use only. Email: Web Site: TABLE OF CONTENTS


Enzyme immunoassay (EIA) permits the routine quantitative determination of many
steroid hormones in body fluids and provides an accurate, sensitive, reproducible, rapid
and specific assay. This enzyme immunoassay method makes it possible to measure
very low concentration of Progesterone in small volumes of serum (0.025 mL per well).

Progesterone is a steroid hormone, which plays an important role in the preparation for
and maintenance of pregnancy. It is synthesized from cholesterol via pregnenolone then
rapidly metabolized to pregnanediol, for the most part, in the liver. 1,8,10 The ovary and
placenta are the major production sites, but a small amount is also synthesized by the
adrenal cortex in both men and women.
Circulating Progesterone levels, which are characteristically low during the follicular
phase, increase sharply during the luteal phase of menstrual cycles, reaching a maximum
some 5 to 10 days after the mid-cycle LH peak.9 Unless pregnancy occurs, a steep
decline to follicular levels sets in about 4 days before the next menstrual period. This
pattern constitutes the rationale behind the well-established use of serum Progesterone
measurements as a simple and reliable method for ovulation detection. 2,3,13
There is growing literature on luteal phase defects. 4,7,16,14,15 Daily Progesterone levels are
considered the most accurate means for documenting a defective luteal phase.4,7
However, some investigators have found that three samples 17 or even a single sample
11,13 (if well timed) can provide valuable information on the adequacy of the luteal phase.
Measurements of serum Progesterone have also been used to check the effectiveness of
ovulation induction, 13 to monitor Progesterone replacement therapy 5 and to detect and
evaluate patients at risk for abortion during the early weeks of pregnancy. 5,6,12 On the
other hand, although Progesterone levels increase throughout pregnancy, they are not
considered a suitable means for monitoring fetal well-being during the third trimester.8

The ANOGEN Coated well immunoenzymatic assay for the quantitative measurement of
serum Progesterone utilizes a monoclonal Anti-Progesterone and a Progesterone-HRP
conjugate. The assay sample and the assay buffer are incubated together with anti-
Progesterone antibody coated plate for sixty minutes and washed. Then the diluted
Progesterone-HRP conjugate is incubated. After the incubation period, the wells are
decanted and washed three times.
The wells are then incubated with a substrate for the enzyme. The product of the enzyme-
substrate reaction forms a blue colored complex. Finally a stopping solution is added to
stop the reaction, which will turn the solution to yellow.
The intensity of color is measured spectrophotometrically at 450 nm in a microplate
reader. The intensity of the color is inversely proportional to the Progesterone
concentration since Progesterone from sample and Progesterone-HRP conjugate
compete for the Anti-Progesterone antibody binding sites. Since the number of sites is
limited, as more sites are occupied by Progesterone from the sample, fewer sites are left
to bind Progesterone-HRP conjugate.
Standards of known Progesterone concentrations are run concurrently with the samples
being assayed and a standard curve is plotted relating the intensity of the color optical
density (O.D) to the concentration of Progesterone. The unknown Progesterone
concentration in each sample is interpolated from this curve.


1. Reliable and reproducible results will be obtained when the assay procedure is carried
out with a strict adherence to the exact procedure described within this package insert and good laboratory practice. 2. Progesterone determination is for diagnostic purposes. The Progesterone concentration should be used only as an adjunct to other data (ex.: results of other tests, clinical impressions, etc.) available to the physician who can take into consideration the history of the patient. Each laboratory should compile its own normal ranges, if possible.
3. This kit is suitable for use with serum of human origin only.
4. A maximal total pipetting time of ten (10) minutes per run is suggested.

Rev. (06/04) PROG-D
All reagents provided are stored at 2-8°C. Refer to expiration date on the label.
96 tests
96 wells
Pre-coated wells with anti-Progesterone Monoclonal Antibody immobilized into the well. 2. CONJUGATE (Part PNEL-2)
0.6 mL
Concentrated Progesterone-HRP conjugate in stabilizer solution. 3. ASSAY BUFFER (Part PNEL-3)
11 mL
Glycine buffer and 0.09% sodium azide as preservative.
4. STANDARD100 ng/L
0.5 mL
Prepared with human Progesterone in human serum containing 0.09% of sodium azide as preservative.
5. STANDARD50 ng/L
0.5 mL
Prepared with human Progesterone in human serum containing 0.09% of sodium azide as preservative.
6. STANDARD20 ng/L
0.5 mL
Prepared with human Progesterone in human serum containing 0.09% of sodium azide as preservative. 1. STANDARD5 ng/L (Part PNEL-7)
0.5 mL
Prepared with human Progesterone in human serum containing 0.09% of sodium azide as preservative.
0.5 mL
Prepared with human Progesterone in human serum containing 0.09% of sodium azide as preservative. 8. STANDARD0.25 ng/L
0.5 mL
Prepared with human Progesterone in human serum containing 0.09% of sodium azide as preservative.
9. STANDARD - 0 ng/L
1 mL
Prepared with human Progesterone in human serum containing 0.09% of sodium azide as preservative. 10. CONJUGATE DILUENT (Part PNEL-10)
11 mL
HEPES buffer pH 7.2 containing thimerosal as a preservative. 11. SUBSTRATE (Part PNEL-11)
11 mL
Buffered solution with TMB.
100 mL
Concentrated solution of saline phosphate buffer with thimerosal as a preservative. Dilute each bottle to one (1) liter with deionized or distilled water.
25 mL
0.5 M Sulfuric Acid (H2SO4). CAUTION: Caustic Material!

Sodium azide may react with lead and copper to form explosive azides.
Flush with copious quantities of water.


1. Precision pipettes (25 µL) with disposable tips or a SMI pipette
2. 8 channels pipette (50 and 100 µL) with disposable tips
3. Plate shaker (100 ± 10 rpm)
4. Microplate reader with filter 450 nm
5. 8 channels repeater pipette for the washing step or multichannel pipette
6. Deionized or distilled water
7. Absorbent paper
1. All materials in this kit may be used only for in vitro clinical or laboratory tests not involving internal or external administration of the material to human or animals. 2. The Standards contain products derived from human blood. Handle the materials as though they were capable of transmitting infectious diseases, since no known test method can offer absolute assurance that such products will not transmit infectious agents even tested non-reactive. 3. Reagents are matched in each kit and therefore reagents from different lot numbers should not be mixed. Do not use after the expiration date. 4. Optimal results will be obtained by strict adherence to this protocol. Respect 4. The kit containing sodium azide and thimerosal as preservatives. These are toxic and therefore all reagents should be handled carefully to avoid ingestion or skin contact. 5. The stopping solution contains sulfuric acid. This solution should be handle with 6. Prior to assay, warm all reagents to ambient temperature by allowing them to stand at room temperature. Gently mix all reagents.
Rev. (06/04) PROG-D
1. Washing solution: dilute each bottle to 1 liter of deionized or distilled water.
2. Diluted PROGESTERONE-HRP conjugate: just before use, dilute the concentrated
Progesterone-HRP conjugate (EC-94051) with Conjugate Diluent (ED-94051) in the proportion of 1 volume of Progesterone-HRP in 20 volumes of Conjugate Diluent. (Dilution 1/21). Since the diluted Progesterone-HRP conjugate is not stable, prepare just enough for the required number of tests. Suggested volume of diluted Progesterone-HRP per number or wells: Volume (mL) concentrated
Volume (mL) Conjugate

Discard unused portion of diluted Progesterone-HRP conjugate after completing the
addition of the reagent to the wells.

Serum must be used in this PROGESTERONE procedure. No additives or preservatives
are necessary to maintain the integrity of the specimen. Store at 2-8°C and assay within
one week after collection. If the assay cannot be performed within one week, freezing is
DO NOT INTERCHANGE REAGENTS BETWEEN KITS BEARING DIFFERENT LOT ALL REAGENTS AND PATIENT SAMPLES SHOULD BE BROUGHT TO 22 ± 2°C ALL REAGENTS AND PATIENT SAMPLES SHOULD BE MIXED BY SWIRLING OR GENTLY VORTEXING. DO NOT INDUCE FOAMING. Refer to the assay procedure, Table I 1. Pipette 25 µL of standard, control or patient sample into the corresponding wells. 2. Pipette 100 µL of the Assay Buffer into each wel . 3. Incubate for sixty (60) minutes on the plate shaker (100 ± 10 rpm) at room 4. Wash manually, precautions must be taken to avoid cross-contamination between wells. Decant the well contents by inverting the plate over a container and without re-inverting, blot the plate against absorbing paper. Wash each well three times with 300 µL of washing solution. At the last wash, decant completely the washing solution by tapping the plate against absorbing paper until no trace of water is visible on the paper. 5. Pipette 100 µL of diluted Progesterone-HRP conjugate in each well. For the dilution procedure see section VII, Reagent preparation. 6. Incubate for thirty (30) minutes on the plate shaker (100 ± 10 rpm) at room 7. Decant and wash the wells three times with 300 µL of washing solution as in step 4. 8. Pipette 100 µL of the TMB enzyme substrate solution to each well. 9. Incubate for twenty (20) minutes on the plate shaker (100 ± 10 rpm) at room 10. Add 50 µL of a stopping solution and shake the microplate to homogenize. 11. Measure the absorbance at 450 nm using a microplate reader NOTE: READ THE ABSORBANCES IMMEDIATELY AFTER COMPLETING THE ASSAY. Wells Identification u
1. Incubate 60 minutes on the plate shaker (100 ± 10 rpm) at room temperature (22 ± 2. Wash 3 times (refer to the washing procedure) 3. Incubate 30 minutes on the plate shaker (100 ± 10 rpm) at room temperature (22 ± 4. Incubate 20 minutes on the plate shaker (100 ± 10 rpm) at room temperature (22 ± CALCULATION OF RESULTS
Examine date for acceptance consistency with quality control guidelines. Aberrant values
may be rejected.
Refer to the sample data and calculations, Table II
For each standard, control and unknown sample, the optical density values are On millimeter paper using the ordinate for the optical density (or the %B/B0) and the abscissa for the standard concentrations (ng/mL), a smooth standard curve is plotted. The values of the control and of unknown samples are read directly from the standard

Results of a typical standard run are shown below:
Progesterone Concentration (ng/mL)

1. SENSITIVITY: The sensitivity of the assay, as defined by the amount of Progesterone
standard that will reduce the maximal binding (Bo = 100% of the Standard 0) by 5%, is 0.2 ng/mL.

a) Intra-assay variation: The precision of the assays was verified by assaying 10
replicates of 3 different sera. The results were: Parameters
b) Inter-assay variation: Reproducibility of the protocol was established by assaying
3 different sera in replicates in successive runs. The results were: Parameters

3. ACCURACY: Known amounts of Progesterone were added to a human serum sample
to determine recovery performance of the assay. The data obtained are below: Expected value
Observed value

4. LINEARITY: 3 serum samples were diluted and run. The results were:
1/2 21.9 13.4 13.9 1/4 10.3 6.9 7.7 1/8 4.9 3.5 3.7
5. SPECIFICITY: Results are expressed as the ratio of Progesterone concentration to
the concentration of the cross- reactant. CROSS-REACTANT %
6. CORRELATION STUDY: Clinical samples were analyzed by the ANOGEN
PROGESTERONE EIA kit in parallel with a similar method. The results of this study are as follows: REGRESSION LINE FROM CORRELATION STUDY

6. EXPECTED NORMAL VALUES: It is recommended that, as with any assay, expected
values for given populations be determined by each laboratory over a suitable period of time and in a statistically significant number of assays before definitive clinical significance is attached to the results of the assay. However, the following may be used as a guide to preliminary interpretation. Females: Follicular Phase
Pregnant Females:


Good laboratory practice requires that quality control specimens be run with each
calibration curve to check the assay performance. Commercial controls are suitable. Any
material used should be assayed repeatedly to establish mean values and acceptable
ranges to assure proper performance.

1. Aufrere MB, Benson H. Progesterone: an overview and recent advances. J Pharm Sci
2. Bauman J. Basal body temperature: unreliable method of ovulation detection. Fertil 3. Brown JB. Timing of ovulation. Med J Austral 1977;2:780-3 4. Gautray JP et al. Clinical investigation of the mentrual cycle: clinical,endometrial and endocrine aspects of luteal defect. Fertil Steril 1981;35:296-303 5. Hensleigh PA, Fainstat T. Corpus luteum dysfunction: serum progesterone levels in diagnostis and assessment of therapy for recurrent and threatened abortion. Fertil Steril 1979;32:396-9 6. Hernandez Horta JL, et al Direct evidence of luteal insufficiency in women with habitual abortion. Obstet Gynecol 1977;49:705-8 7. Jones G. luteal phase defects. In: Behrman SJ, Kistner RW, editors. Progress in infertility. Boston: Little, Brown and company. 2nd ed, 1975:299-324 8. Klopper A, Fuchs F. Progestagens. In: Fuchs F, Klopper A, editors. Endocrinology of Pregnancy. Hagerstown: Harper&Row, 1977:99-122 9. March CM. Goebelsmann U, Nakamura RM, Mishell DR. Roles of estradiol and progesterone in eliciting the midcycle luteinizing hormone and follicle-stimulating hormone surges. J Clin Endocrinol Metab 1979;49:507-13 Progesterone, A BIO-ED TM slide/seminar educational program. Rochester: Bioeducational Publications, 1981 11. Radwanska E, et al. Plasma progesterone and oestradiol estimations in the diagnostis and treatment of luteal insufficiency in menstruating infertile women. Acta Eur Fertil 1976;7:39-47 12. Radwanska E, et al. Plasma progesterone levels in normal and abnormal early human pregnancy. Fertil Steril 1978;30:398-402 13. Radwanska E, et al. Single midluteal, progesterone assay in the management of ovulatory infertility. J Reprod Med 1981;26:85-9 14. Sheehan KL, Casper RF, Yen SSC. Luteal phase defects induced by an antagonist of luteinizing hormone-releasing factor: a model for fertility control. Science 1982;215:170-2 15. Wentz A. Pathophysiology of luteal phase inadequacy. In: Tozzini RI, Reeves G, Pineda RL, editors. Endocrine Physiopathology of the ovary. Amsterdam: Elsevier/North Holland, 1980:257-74 16. March CM. Luteal phase defects. In: Mishell DR, Davajan V, editors. Reproductive Endocrinology, Infertility and Contraception. Philadelphia: F.A. Davis Company, 1979:469-76 17. Abraham GE. The application of natural steroid radioimmunoassay to gynecologic endocrinology. In: Abraham GE, editor. Radioassay Systems in Clinical Endocrinology. Basel: Marcel Dekker, 1981:475-529


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