Rabbit anti-CD163 Cat. No. and Size: 503-3964: 1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. 503-3961: 7.0 ml pre-diluted rabbit monoclonal antibody purified by protein A/G in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. Intended Use:
For research use only. Not for use in diagnostic procedures.
Immunogen:
Synthetic peptide corresponding to a region near N-terminus of human CD163 protein
Isotype: Epitope: Molecular Weight: Species Reactivity:
Human (tested). Bovine, pig (by sequence homology). Others not tested.
Description:
CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant
Applications
Immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissue sections
IHC Procedure Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 1 mM EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: 30 minutes at RT. IHC Positive Control: Cellular localization: Storage and Stability Store at 2-8°C. Do not freeze. The user must valida te any other storage conditions. Every antibody is expiration dated. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. - For Research Use Only -
Explanation of the symbols on the product label:
Consulter les instructions d'utilisation
Zytomed Systems GmbH • Anhaltinerstraße 16
Dispositif médical de diagnostic in vitro
14163 Berlin, Germany • Tel: (+49) 30-804 984 990
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